Interleukin-6 from the Adipose Secretome Potentiate Differentiation of Adipose Progenitors Through Activation of Redox Signaling

<b>Supplementary Figure 1. Adipogenic Stimulus Increases the Number of Short Mitochondria.</b> (A) Box plot of mitochondrial length analyzed by category, short, intermediate, or elongated for APCs treated adipogenic stimulus or vehicle normalized to cell number per slide in mitotracker-stained APCs (B) Representative images of mitotracker stained APCs.<b>Supplementary Figure 2. Conditioned Media Treatment Increases Adipogenesis.</b> (A) Eluted Oil Red O absorbance for cells treated with CD CM or HFD CM from male subcutaneous adipose tissue explants or vehicle on day 7 of differentiation (n=4). * p ≤ 0.05.<b>Supplementary Figure 3. IL-6 and TNF Identified as Upstream Regulators </b>(A) Network of IL-6 as an upstream regulator of differentially expressed proteins found in our dataset in IPA. (B) Network of TNFa as an upstream regulator of differentially expressed proteins found in our dataset in IPA.<b>Supplementary Figure 4. IL-6 Treatment Increases Adipogenesis.</b> (A) Eluted Oil Red O absorbance from APCs treated with IL-6 [0.1, 1, 5 ng/mL] (n=4). * p ≤ 0.05, ** p ≤ 0.01; *** p ≤ 0.001, **** p ≤ 0.0001.<br>