Effects of m6A modification and YTHDF2 knockdown on global mRNA turnover

We developed a method to deconvolute decays of m6A mRNAs and their unmethylated isoforms from total RNAs across the transcriptome and combined this method with the knockdown of m6A reader protein to study the effect on global mRNA turnover. M6A-RIP-seq approach was combined with time-course experiments to monitor total RNA, m6A mRNA and unmethylated isoform mRNA decays across the transcriptome upon DF2 knockdown by two different DF2 siRNAs. Knockdown with a control siRNA was used as a negative control. A total of fifty-four RNA samples was generated.