A single cell atlas of mouse embryonic diapause and peri-implantation development

Here, we molecularly defined embryonic diapause at single-cell resolution, revealing hidden transcriptional dynamics while seemingly the embryo resides in a state of suspended animation. Alongside, we found that the dormant pluripotent cells are aware of their microenvironment which sustains their viability via Yap-mediated mechanotransduction. Approximately 200~250 blastocysts from each developmental stage E4.5, EDG7.5, EDG9.5 and Reactivated and 50 embryos from E5.5 stage were collected and analyzed using sc-RNA seq.本研究以单细胞分辨率对胚胎滞育（embryonic diapause）进行了分子层面的解析，揭示了看似处于休眠停滞状态的胚胎所隐藏的转录动态变化。与此同时，我们发现休眠状态的多能细胞能够感知自身所处的微环境，并通过Yap介导的机械转导通路维持自身存活活性。本研究收集了E4.5、EDG7.5、EDG9.5各发育阶段以及激活状态的囊胚约200~250个，同时收集E5.5阶段胚胎50个，并采用单细胞RNA测序（sc-RNA seq）对上述样本开展了分析。