Puccinia striiformis f. sp. tritici PST-130 genome sequencing project. Puccinia striiformis f. sp. tritici PST-130

DNA extracted from spores of one race is being sequenced. We used Illumina sequencing to rapidly access the genomic sequence of the highly virulent PST race 130. We obtained nearly 80 million high quality paired-end reads (>50x coverage) that were assembled into 29,189 contigs (64.8 Mb), which provide an estimated coverage of at least 88% of the PST genes. The assembled contigs provided an inventory of PST repetitive elements, which were annotated and deposited in Repbase.