Brain-infiltrating ILC2s-derived alpha-calcitonin gene-related peptide promoting angiogenic sprouting accelerates post-stroke angiogenesis

Group 2 innate lymphoid cells (ILC2s) are key players in immune regulation and tissue repair in barrier immunity, but they are rarely seen in the brain. Since ILC2s can be intrinsically expanded by IL2/IL2 antibody complex, we investigated the transcriptomic changes induced by IL2/IL2 antibody complex (IL2-JES6-1) after ischemic stroke, we performed single-cell RNA sequencing of the CD45high immune cells from the infarcted hemisphere at day 14 after MCAO using 10x Genomics scRNAseq. To investigate the key mechanism of ILC2s-mediated long-term recovery after ischemic stroke, we performed single-cell RNA sequencing of the infarcted hemisphere at day 14 after MCAO using 10x Genomics scRNAseq. Single-cell RNA profiling of brain CD45high immune cells (treated with IL2/IL2Ab complex or PBS) or whole brain cells in MCAO mice (treated with ILC2s or PBS) a 14 dpi.