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Precise genome editing via one homology-arm mediated gene integration

NIAID Data Ecosystem2026-05-25 收录

基因敲入技术

基因组编辑

数据链接：

https://www.ncbi.nlm.nih.gov/sra/SRP126448 数据链接链接失效反馈

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资源简介：

Establishment of precise targeted gene knock-in technology

应用场景：

创建时间：

2018-06-28

基因组编辑

植物科学

Polyploidy or whole-genome duplication (WGD) is a significant evolutionary force. However, the mechanisms governing polyploid genome evolution remain unclear, limited largely by a lack of functional

DataONE2025-08-13 更新220

Additional file 9: of NmeCas9 is an intrinsically high-fidelity genome-editing platform

基因组编辑

脱靶效应分析

Table S7. Primer sequences used for on- and off-target deep sequencing analysis for all genomic editing sites analyzed by GUIDE-seq. (XLSX 28 kb)

Figshare2024-02-08 更新80

Additional file 3: of CRISPRO: identification of functional protein coding sequences based on genome editing dense mutagenesis

基因功能验证

基因组编辑

Table S2, Annotated CRISPRO output (XLSX 33034 kb)

Figshare2018-10-20 更新80

RefSeq_Gorilla_modified_THRA2

基因组编辑

基因表达

This dataset was used to quantify expression levels of THRA isoform 1 and THRA isoform 2. Given that THRA2 had not been deposited yet, we edited the UCSC Kamilah gorilla genome GGO, version 6 to inclu

DataCite Commons2023-11-02 更新160

Survey of 3 prime GG gRNA sites in 8 genomes

基因组编辑

gRNA

gRNA sites with a 3' GG motif adjacent to the PAM site have been shown to exhibit substantially higher genome-editing efficiency in C. elegans. I scanned 6 model genomes (Saccharomyces cerevisiae, Cae

DataCite Commons2020-09-04 更新80

Precise genome editing via one homology-arm mediated gene integration

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