Number of GABAergic activated neurons in Control, Paradoxical sleep deprived or hypersomniac rats.

Number (mean��sem) of Fos+ and Fos-GAD neurons counted in the brainstem in PSC, PSD and PSR rats on a total of 13 sections at 600 ��m intervals through the full rostro-caudal extent of the pons and medulla after Fos immunohistochemistry combined with GAD67 mRNA in situ hybridization. The values displayed are an average across 4 animals in each group of the sum of all Fos+ neurons (Fos+Total) and Fos/GAD67 double-labeled neurons (Fos+/GAD+) counted on one or several sections (column n) depending on the rostrocaudal extent of the structures. %GAD/Fos indicates the percentage of Fos+ neurons that are GABAergic. Significance values indicated for individual points are: *P#P##P###P