Gene expression profile at single cell level of neuroblastoma tumors and their tumor microenvironment

Neuroblastoma (NBL) tumors are considered heterogeneous tumors. So far, little detailed information available on their immune environment. We used single cell RNA sequencing (scRNA-seq) to analyze the tumor microenvironment of neuroblastoma. Fresh neuroblastoma tumor samples (n=10 before treatments, n=14 after induction chemotherapy) were enzymatically digested and single-cell FACS sorted into plates for scRNA-seq. NOTE FROM SUBMITTER: raw data can not be included due to patient privacy concerns