We find that pre-crRNAs comprising a full-length direct repeat (full-DR-crRNA) sequence with specific stem-loop G-C base substitutions exhibit increased editing efficiencies compared with the standard

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DNA methylation analyses of Hyg R colonies obtained in cotransformations of N644 with hH3 alleles.

基因编辑效率

DNA甲基化分析

1Fraction transformations that gave rise to HygR colonies. 2Fraction transformations that showed loss of DNA methylation in Southern blotting. 3One transformant out of 12 tested exhibited hypomethylat

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Phosphonoacetate Modifications Enhance the Stability and Editing Yields of Guide RNAs for Cas9 Editors

CRISPR引导RNA

基因编辑效率

CRISPR gene editing and control systems continue to emerge and inspire novel research and clinical applications. Advances in CRISPR performance such as optimizing the duration of activity in cells, ti

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Additional file 2 of Increased prime edit rates in KCNQ2 and SCN1A via single nicking all-in-one plasmids

基因编辑效率

癫痫相关基因

Additional file 2: Table S1. Individual data values of all data represented in this manuscript.

Figshare2023-07-14 更新30