Mapping-by-sequencing of the pod2 mutation

To identify the mutation that underlies the POD2 locus, we performed mapping-by-sequencing on an F2 population derived from the cross between an pod2 mutant and a wild tomato Solanum pimpinellifolium plant (accession LA1589). Genome-wide analysis of the allele frequencies in two pools containing 16 mutant and 25 randomly selected wild-type plants allowed us to identify the genomic region harboring the causal mutation of the pod2 phenotype.