Investigating the Blocking Effect of Alkanethiol Self-assembled Monolayer on Electrochemical Response of the Split Gq-based DNA-NTs-based Biosensor (Supporting Information)

The split G-quadruplex-based DNA-nanotweezwers-based electrochemical DNA sensor was characterized by focusing on the blocking ability of the mixed self-assembled monolayer (SAM) against the nonspecific adsorption of hemin and the direct reduction of oxygen on a gold electrode. We found that the mixed SAM composed of MCH and MHA (concentration of each alkanethiol in SAM formation solution was 1.0 mM : 0.1 mM) effectively suppressed the nonspecific adsorption of hemin and the direct reduction of oxygen on the gold electrode.

本项研究针对基于G-四链体结构的DNA纳米镊子型电化学DNA传感器进行了表征，其重点在于混合自组装单分子层（SAM）对血红素非特异性吸附以及金电极上氧的直接还原的抑制能力。研究发现，由MCH和MHA（在SAM形成溶液中每种烷硫醇的浓度为1.0 mM : 0.1 mM）组成的混合SAM能够有效抑制血红素的非特异性吸附以及金电极上氧的直接还原反应。