A global transcriptome analysis of human epidermal keratinocytes upon knockdown of hsa_circ_0084443

Hsa_circ_0084443 expression level is down-regulated during normal skin wound healing and higher level of hsa_circ_0084443 was found in chronic non-healing diabetic foot ulcers compared to normal wounds. However, the biological function of hsa_circ_0084443 in epidermal keratinocytes during wound repair has not been studied. To study the genes regulated by hsa_circ_0084443, we transfected siRNA targeting hsa_circ_0084443 diagnostic junction into human primary epidermal keratinocytes to knockdown hsa_circ_0084443 expression. We performed a global transcriptome analysis of keratinocytes upon knockdown of hsa_circ_0084443 using Affymetrix arrays. Expression profiling of human primary epidermal keratinocytes transfected with 50 nM si-hsa_circ_0084443 or si-Control for 24 hours (biological triplicates in each group) was performed using Affymetrix GeneTitan system.