Genetic and pharmacological evidence for kinetic competition between alternative poly(A) sites in yeast - NNS complex
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下载链接：
https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE160567
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资源简介：
PAT-seq approach was used to determine changes to 3'UTR length in yeast upon mutation of NNS subunits Nrd1 (nrd1-5), Nab3 (nab3-11) and Sen1 (sen1-1) compared to wild type cells Yeast cells were grown at 25ºC until an OD600 of 0.6 was reached then transferred to 37ºC for 1 hour before harvesting. Samples were analysed via PAT-seq 3'UTR focused deep-sequencing in replicate using the Illumina Hiseq1500 platform.
创建时间：
2021-07-20
