SHC1 mediates cytokine-induced phosphorylation of GAB2

Binding of Gab2 to tyrosine phosphorylated Shc promotes the phosphorylation of Gab2 by an unknown kinase. Gab2 becomes tyrosine phosphorylated in response to IL-2 (Brockdorff et al. 2001) and IL-3 (Gu et al. 1998). Chimeric receptors were used to demonstrate that Shc is sufficient for Gab2 tyrosine phosphorylation. In response to IL-3, Grb2 was also required, reflecting that Gab2 is recruited to the activated cytokine receptor complex as a complex of Gab2:Grb2 (Gu et al. 2000).

Gab2与酪氨酸磷酸化Shc的结合促进未知激酶对Gab2的磷酸化。Gab2在IL-2（Brockdorff等，2001年）和IL-3（Gu等，1998年）的刺激下发生酪氨酸磷酸化。通过使用嵌合受体，证明了Shc对于Gab2酪氨酸磷酸化是充分的。在IL-3的刺激下，Grb2也成为了必需因素，这反映出Gab2作为Gab2:Grb2复合体被募集到激活的细胞因子受体复合物中（Gu等，2000年）。