Label free quantitative proteomics analysis based on LC-MS in Caki-1 to reveal the pathways associated with ERRα in ccRCC

To investigate the regulatory role of orphan nuclear receptor ERRα in the progression of ccRCC, we conducted the stable knockdown of ERRα in Caki-1 with shRNA lentivirus. The total protein samples of Caki-1 with shCtrl and shERRα were collected and subjected to label free quantitative proteomics analysis based on LC-MS, the differentially expressed proteins were selected based on the p value and fold-changes between two groups. Then, bioinformatics analysis was performed to reveal the pathways and key factors related to ERRα in ccRCC