Analysis of delta-9-tetrahydrocannabinol, cannabidiol and cannabinol

These datasets consist of a gas chromatography-mass spectrometry method for the detection and quantitation of cannabinoids was developed and validated in aqueous and oral fluid matrices.The behaviour of Δ9-tetrahydrocannabinol and cannabidiol was further characterised in terms of the pre-analytical parameters and a significant difference was illustrated between the two matrices. The detection and quantification of cannabinoids within biological matrices are still required, despite the legalisation of cannabis use in South Africa. The development of a fit-for-purpose analytical method for the quantification of cannabinoids in biological matrices other than urine is paramount to accommodate the recent legalisation of cannabis use in South Africa.
The limit of quantitation of 2.0 ± 0.5 ng/mL (at 95% confidence level) for Δ9- tetrahydrocannabinol in oral fluid was lower than the proposed threshold limit of 5 ng/mL instated in other countries. It was concluded that oral fluid has the potential to serve as an alternative matrix to urine when testing for cannabis use but the sampling uncertainty associated with the collection of authentic oral fluid samples has yet to be determined.It was concluded that the more convenient aqueous matrices cannot be used as a substitute for authentic oral fluid during method validations.

本研究数据集包含了一种针对水相和口腔液相介质中大麻素检测与定量的气相色谱-质谱法，其方法已开发并验证。在分析前参数方面，对Δ9-四氢大麻酚和CBD的行为进行了进一步表征，并在两种介质之间展示了显著的差异。尽管南非已将大麻使用合法化，但生物基质中大麻素的检测与定量仍属必要。针对除尿液之外生物基质中大麻素进行适宜用途分析方法的开发，对于适应南非近期大麻使用合法化至关重要。对于口腔液中Δ9-四氢大麻酚的定量限为2.0 ± 0.5 ng/mL（95%置信水平），低于其他国家提出的5 ng/mL的拟议阈值限。结论认为，口腔液具有作为检测大麻使用时尿液替代介质的潜力，但采集真实口腔液样本的采样不确定性尚待确定。研究还得出结论，更便利的水相介质不能作为方法验证期间真实口腔液的替代品。