RNA-seq of coding RNA from single cells derived from mouse embryos

The study was aimed at interrogating the early stages of blood cell development within the embryo. Blood develops from mesoderm, which is specified at gastrulation. We therefore sorted cells from mouse embryos between E6.5 and E7.75 to capture gastrulation and the emergence of mesoderm, followed by specification of the first mature blood cells.本研究旨在解析胚胎内血细胞发育的早期阶段。血液由中胚层（mesoderm）发育而来，而中胚层在原肠胚形成（gastrulation）阶段完成特化。因此，我们对E6.5至E7.75时间段的小鼠胚胎细胞进行分选，以捕获原肠胚形成、中胚层出现以及首批成熟血细胞特化的连续发育阶段。