Metabolic Engineering and Adaptive Evolution of Escherichia coli for Efficient D-Glucaric Acid Synthesis from D-Xylose with Methanol Assistance

D-Glucaric acid is a value-added dicarboxylic acid that can be utilized in the chemical, food, and pharmaceutical industries. Due to the complex process and environmental pollution associated with the chemical production of D-glucaric acid, bioconversion for its synthesis has garnered increasing attention in recent years. In this study, a novel cell factory was developed for the efficient production of D-glucaric acid using D-xylose-methanol mixtures.