RNA-seq for WT and SCID mT3-2D KPC Tumors and mT3-2D cell lines

To investigate how pancreatic malignant epithelial cells responded to anti-tumor T-cell immune responses in vivo, RNA-seq was used to examine the gene expression profiles of the mT3-2D-GFP cell line and FACS sorted mT3-2D-GFP cancer cells isolated from WT and SCID tumors. RNA was extracted from 6 WT tumors GFP+ sorted cells, 5 SCID tumors GFP+ sorted cells, and the mT3-2D-GFP cell line. Equal amounts of RNA from each corresponding group were combined as one sample (1 WT sample, 1 SCID sample, and 1 cell line). Then, RNA-seq was used to examine the gene expression profiles of these samples