Transcriptomic profiles of human foreskin fibroblast cells in response to orf virus. Homo sapiens

Using RNA sequencing technology, we compared specific changes in the transcriptomic profiles in human foreskin fibroblast cells (HFF-1) following ORFV infection. ORFV specifically upregulated and downregulated a variety of genes, including genes involved in antiviral immune response, apoptosis, cell cycle and a series of signaling pathways, such as the IFN and p53-signaling pathways. A range of genes were downregulated at early stage. In contrast, the virus up-regulates a number of cellular genes at late stage. Overall design: HFF-1 mRNA profiles of 0, 3, and 8 hours post infection with orf virus were generated by deep sequencing, in triplicate, using Illumina Hiseq2500.