DamID-based chromatin profiling of dHey in Kc cells

Chromatin binding profiling showed that dHey binds to enhancers in order to maintain EC identity, and co-regulates nuclear organization by governing a switch in the expression of nuclear lamins. Moreover, like dHey loss, de-regulated expression of lamins override identity programs. Thus, a single TF concomitantly orchestrates chromatin state, enhancer activity, and nuclear organization safeguarding differentiated cell identity Chromatin profiling using Dam-dHey shows preferential recruitment to enhancers and transcriptionally active regions. Manuscript title: Specification and maintenance of enterocyte identity requires dHey and nuclear lamins For determaning dHey chromatin profile, Kc cells were transiently transfected with a plasmid encoding Dam_dHey or Dam alone. Two biological replicates (sample 1and 2) and one dye-swap techincal replicate (sample 3) were used.