Transcriptional changes during astrocyte maturation in vivo at single cell resolution

Analysis of gene expression during striatal astrocyte maturation in vivo in mouse by scRNA-Seq In vivo astrocytes were purified from mice using anti-ACSA2 MACS at indicated ages. Cells were analysed by 10X-Genomics scRNA-Seq.采用单细胞RNA测序（scRNA-Seq）解析小鼠体内纹状体星形胶质细胞成熟过程中的基因表达。 研究人员在指定日龄的小鼠体内，采用抗ACSA2磁激活细胞分选（MACS）技术纯化星形胶质细胞。 随后利用10×基因组学（10X-Genomics）平台开展单细胞RNA测序分析。