The role of IL-6 and IL-27 signalling on virus specific CD4 T cell responses during LCMV Cl13 infection. The role of IL-6 and IL-27 signalling on virus specific CD4 T cell responses during LCMV Cl13 infection

LCMV Clone 13 is an arenavirus that results in a persistent viral infection in mice when delivered intravenously. It has been widely used to explore the impact of chronic viral infection on CD4 T cell responses and dissect mechanisms that regulate this. In this study we used mixed bone marrow chimeras from wildtype and cytokine reeptor deficient (gp130 or IL-6/IL-27R) mice to determine the role these cytokines on splenic CD4 T cell responses 30 days post infection Overall design: Mixed bone marrow chimeras of CD45.1+ wildtype and CD45.2+ receptor deficient (either gp130 or IL-6/IL-27R deficient) cells were intravenously administed to irradiated WT mice. 8 weeks later mice were infected with LCMV Clone 13 intravenously. 30 days post infection the spleen was isolated and CD45.1+ and CD45.2+ PD1+CD4+ T cells (for gp130 study) or CD45.1+ and CD45.2+ TFH (for IL-6R/IL-27R study) were FACS isolated. RNA was extracted and transcriptomics performed.