Effect of fipronil on the thyroid gland transcriptome in the rat

Fipronil is a widely-used broad-spectrum phenylpyrazole insecticide. It has been shown that fipronil increases the hepatic metabolism of thyroid hormone in the rat, which may induce compensatory mechanisms at the level of the thyroid gland itself. Here, we studied the effect of fipronil on the transcriptome of the thyroid gland in the rat. Adult (2- to 3-month-old) female Wistar rats were treated per os with fipronil (3 mg/kg) or the vehicle alone for 14 days. The transcriptome of their thyroid gland was analyzed using Agilent 4x44K microarrays.氟虫腈（Fipronil）是一种广泛使用的广谱苯基吡唑类杀虫剂。已有研究表明，氟虫腈可增强大鼠体内甲状腺激素的肝脏代谢，进而可能在甲状腺腺体自身层面诱发代偿机制。本研究探究了氟虫腈对大鼠甲状腺转录组的影响。实验选用2~3月龄的成年雌性Wistar大鼠，经口给予氟虫腈（3 mg/kg）或单独给予赋形剂，连续处理14天。采用安捷伦（Agilent）4x44K基因微阵列芯片对各组大鼠的甲状腺转录组进行分析。