Genome assemblies and sequencing data for two Rhizoctonia isolates, AG8-1 and AG8-3

The fungal pathogen Rhizoctonia solani infects a diverse range of host plants and remains an intractable and economically significant disease for many crops. R. solani is classified into reproductively incompatible anastomosis groups (AGs). In the vegetative stage, most plant-pathogenic R. solani isolates are multinuclear and heterokaryotic, but little was previously known about the diversity between haplotypes due to highly fragmented, collapsed short-read assemblies. We used PacBio HiFi and Hi-C sequencing to achieve fully-phased, chromosome-scale genome assemblies of the broad host-range R. solani isolates AG8-1 and AG8-3. We generated both RNA-seq and PacBio Iso-Seq data to achieve a high-quality gene annotation. This provides a platform to further dissect the mechanisms enabling this globally significant agricultural pathogen to inflict losses to a range of crop hosts.