Inhibition of MGL reduced GSIS in INS-1 cells and isolated rat islets.

(A) JZL184 dose-dependently reduced GSIS during static incubations in INS-1 cells (n = 3). (B) JZL184 (10 μM) reduced GSIS in INS-1 cells regardless of the temporal exposure (n = 3). (C) INS-1 cells demonstrated a partial recovery of GSIS following the removal of JZL184 (10 μM) for 2 hours (n = 3). (D) MJN110 dose-dependently reduced GSIS during static incubations in INS-1 cells (n = 4). (E) URB602 (50 μM) inhibited GSIS during static incubation in INS-1 cells (n = 3). (F) JZL184 dose-dependently reduced GSIS in isolated whole rat islets during a static incubation (n = 3). Error bars represent S.E. from independent experiments (*, p