NGS data of CRISPR/dCas9 activation screen with CDK4/6 inhibitor Palbociclib in bladder cancer
收藏Mendeley Data2020-06-01 更新2026-04-09 收录
下载链接：
https://data.mendeley.com/datasets/ptx3dzs926
下载链接
链接失效反馈官方服务：
资源简介：
We used a genome-scale functional genomics approach to determine the mechanisms of resistance to PD0332991. Transcriptional activation was performed using the synergistic activation mediator (SAM) CRISPR-dCas9 library. Work flow: a) T24 cells with stably transduced SAM components. b) Low MOI lentiviral transduction of amplified sgRNA library. c)antibiotic selection for transduced cells. e) divide cells into control(vehicle) and PD 7 days treatment. f) NGS of PCR amplicons. g) Comparison of sgRNA counts in control and treatment conditions.
创建时间：
2020-06-01
