NGS data of CRISPR/dCas9 activation screen with CDK4/6 inhibitor Palbociclib in bladder cancer
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资源简介：
We used a genome-scale functional genomics approach to determine the mechanisms of resistance to PD0332991. Transcriptional activation was performed using the synergistic activation mediator (SAM) CRISPR-dCas9 library. Work flow: a) T24 cells with stably transduced SAM components. b) Low MOI lentiviral transduction of amplified sgRNA library. c)antibiotic selection for transduced cells. e) divide cells into control(vehicle) and PD 7 days treatment. f) NGS of PCR amplicons. g) Comparison of sgRNA counts in control and treatment conditions.
创建时间：
2020-06-01
