rab-10(wy787) causes defects in PVD dendritic morphology.

(A, B) PVD was labeled with a cell-specific marker (ser2prom3::myrGFP). The dendritic arbor covers the entire body of a wild-type animal. 2° and 3° branches are established by the L3 larval stage, and by the L4 larval stage the dendritic arbor has fully elaborated with 4° branches. A second cell body appears in some micrographs because ser2prom3::myrGFP also labels the non-branching PDE neuron. (C, D) wy787 mutants have severely disrupted dendritic morphology. 2° branches emerge in the L3 stage but most do not reach the 3° branch-point or extend 3° branches. Full menorah form only in the distal anterior region of the dendrite. (E) Schematic of the PVD dendritic arbor in wild-type and wy787 animals. (F) Quantification of 2° branch filopodia in larval stages in wild-type and wy787 animals. (G) Definition of branch complexity index used to quantify menorah completeness per region for each genotype. (H) Quantification of subcellular distribution of branch complexity in wild-type and wy787 animals. Scale bars represent 20 μm. Error bars represent SEM. * p5 for all genotypes.