Protocols for inducible recombination in TPH2-tTA/Ptet-Cre/R26R mice.

Dox was administered with 5 µg/ml from initiation of matings until birth. At P0, Dox concentration was changed to 50 µg/ml. In protocol 1, transgenic mice were maintained in the absence of Dox throughout life until sacrifice at P90. In protocol 2, mice were maintained under chronic Dox administration throughout life until sacrifice at P90. In protocol 3, Dox was given from conception until P0 when it was withdrawn until sacrifice at P90. In protocol 4, Dox was given from conception until P60 when Dox was withdrawn until sacrifice at P90. +Dox, administration of Doxycycline; θDox, no administration of Dox.