RNA sequencing of allelic series of miR-17~92 mutant embryos

We report the generation of an allelic series of miR-17~92 mutant mice, each carrying the targeted deletion of specific miRNA families encoded by the cluster. We integrate the phenotypic characterization of these strains with RNA-seq analysis showing the consequences of selective miRNA inactivation on gene expression in the developing embryo. We generated and analyzed RNA-seq data from 8 different miR-17~92 alleles, in three different microdissected tissues collected from E9.5 mouse embryos. Datasets contain biological triplicates (except for duplicates in one case, Delta_17_18_92) for a total of 71 samples.本研究报道了miR-17~92等位基因系列突变小鼠的构建，每株小鼠均携带该基因簇编码的特定微小RNA（miRNA）家族的靶向缺失。我们将这些品系的表型表征与RNA测序（RNA-seq）分析相结合，揭示了选择性miRNA失活对发育中胚胎基因表达的影响。我们针对采集自胚胎期第9.5天（E9.5）小鼠胚胎的三种显微切割组织，生成并分析了对应8种不同miR-17~92等位基因的RNA-seq数据。本数据集包含生物学重复三次的样本（仅Delta_17_18_92组为生物学重复两次），总计71份样本。