Single-cell Sequencing of adipose Treg cells

Systematic characterizations of adipose Treg subsets and their in vivo roles remain uncommon. Using paired single-cell RNA-seq and TCR-seq to map adipose Treg cells, we identified conserved adipose subsets with distinct clonal expansion patterns. By gain and loss of function studies, we demonstrated adipose Treg subsets display disparate influences. To understand the clonal structure of adipose Treg cells, we analyzed their TCR sharing data and observed a number of overlapped TCR clonotypes, suggesting a cellular state transition between adipose Treg clusters. Our findings have important implications for understanding phenotypic diversity of tissue Tregs in vivo. Overall design: Around 30,000 TCRß+CD4+CD25+YFP+ adipose Treg cells from Foxp3Cre mice were sorted by flow cytometry into a chilled tube filled with 30µl PBS with 0.04% BSA. Cells were then encapsulated in one lane of a 10x Chromium instrument, Libraries were constructed with a Single Cell 5' Reagent Kit. And TCR transcripts can be enriched with a Single Cell V(D)J Reagent Kit. We obtained 14,274 good quality cells after filtering. We first generated a resource on the gene expression profiles of murine adipose Treg cells.