Dissecting the mechanisms of environment sensitivity of smart probes for quantitative assessment of membrane properties

General Description

It contains data sets from Ragaller et al, Open Biology, 2022 (https://doi.org/10.1098/rsob.220175).

It contains Generalized Polarization (GP) values, GP profiles, GP maps, raw spectral images, MD Simulation snapshots, tilt angle distributions, partial density profiles, relaxation time and stokes shift

Abstract

The plasma membrane serves as a crucial platform for a multitude of cellular processes. Its collective biophysical properties are largely determined by the structural diversity of the different lipid species it accommodates. Therefore, a detailed investigation of biophysical properties of the plasma membrane is of utmost importance for a comprehensive understanding of biological processes occurring therein. During the past two decades several environment-sensitive probes have been popular tools to investigate membrane properties. Although these probes are assumed to report on membrane order in similar ways, their individual mechanisms remain to be elucidated. In this study, using model membrane systems, we studied the probes Pro12A, NR12S and NR12A in depth and examined their sensitivity to parameters with potential biological implications, such as the degree of lipid saturation, double bond position and configuration, phospholipid headgroup and cholesterol content. Applying spectral imaging together with atomistic molecular dynamics simulations and time-dependent fluorescent shift analyses, we unravelled individual sensitivities of these probes to different biophysical properties, their distinct localizations and specific relaxation processes in membranes. Overall, Pro12A, NR12S and NR12A serve together as a toolbox with a wide range of applications allowing to select the most appropriate probe for each specific research question. 

Data usage

Researchers  are welcome to use the data contained in the dataset for any projects.  Please cite this item upon use or when published. We  encourage reuse using the same CC BY 4.0 License.

Data Content

Excel files for graphs
Microscopy Images

Software to open files

.csv - Microsoft Excel
.tif, .lsm - Fiji (https://imagej.net/Fiji.html#Downloads)
.pzfx - GraphPad Prism
.svg - Inkscape (https://inkscape.org/)

{'General Description': '本数据集汇集了Ragaller等人在《开放生物学》杂志上发表的2022年数据集（https://doi.org/10.1098/rsob.220175）。其中包含了一般极化（GP）值、GP轮廓、GP地图、原始光谱图像、分子动力学模拟快照、倾斜角度分布、部分密度轮廓、弛豫时间和斯托克斯位移。', 'Abstract': '质膜作为众多细胞过程的至关重要的平台，其整体生物物理性质在很大程度上由其所容纳的不同脂质种类的结构多样性所决定。因此，对质膜生物物理性质进行详尽的研究，对于全面理解其中的生物过程至关重要。在过去二十年中，多种环境敏感探针已成为研究膜性质的热门工具。尽管这些探针被认为以类似的方式报告膜秩序，但其个体机制仍有待阐明。在本研究中，我们利用模型膜系统，对探针Pro12A、NR12S和NR12A进行了深入研究，并考察了它们对具有潜在生物学意义的参数的敏感性，如脂质饱和度、双键位置和构型、磷脂头部基团和胆固醇含量。通过光谱成像结合原子分子动力学模拟以及时间依赖性荧光偏移分析，我们揭示了这些探针对不同生物物理性质的个体敏感性、它们在膜中的独特定位以及特定的弛豫过程。总体而言，Pro12A、NR12S和NR12A共同构成了一个工具箱，具有广泛的应用范围，允许针对每个具体的研究问题选择最合适的探针。', 'Data usage': '研究人员欢迎使用数据集中包含的数据进行任何项目。请在使用或发表时引用此项目。我们鼓励使用相同的CC BY 4.0许可进行重用。', 'Data Content': '用于图表的Excel文件
显微镜图像
打开文件所需的软件
.csv - Microsoft Excel
.tif, .lsm - Fiji（https://imagej.net/Fiji.html#Downloads
.pzfx - GraphPad Prism
.svg - Inkscape（https://inkscape.org/）'}