RNA-seq analysis of rat fetal testis following in utero dipentyl phthalate exposure

RNA-seq analysis was conducted as part of an experiment investigating the effects of the phthalate, dipentyl phthalate (DPeP), on the rat fetal testis. The goal of the study was to test what gene expression responses occurred in a dose-dependent fashion, and which were most sensitive, following exposure to 1, 11, 100, or 300 mg/kg/d DPeP from gestational day (GD) 17-21. Timed pregnant female Sprague Dawley rats were treated with 0 (corn oil vehicle), 1, 11, 100, or 300 mg DPeP/kg/d in a 2.5 mL/kg dosing solution, by oral gavage from GD 17-21. Fetal testes were collected on GD 21. Each biological replicate consists of total RNA extrated from 1 testis or 2 pooled testes from the same litter (pregnant dam).