ChIP-seq of Escherichia coli RpoS during stationary phase. ChIP-seq of Escherichia coli RpoS during stationary phase

To determine sites where RpoS binds (and hence likely plays a direct role in transcription), we used ChIP-seq to map the association of RpoS across the Escherichia coli chromosome during stationary phase growth in minimal medium. To facilitate ChIP, RpoS was C-terminally SPA-tagged at its native locus.