Translational Reprogramming as a Driver of Antimony-drug Resistance in Leishmania

Two types of changes were examined at the translatome level in Leishmania: changes at the translational level in drug-resistant strain occurring in the absence of the drug (general basal changes), and those only detectable when the resistant parasites are growing under SbIII exposure (changes to combat the drug). Supplementary files description: File name: Supplementary Data 1 Description: Raw data of automatic polysome fractionation per experimental condition. File name: Supplementary Data 2: Description: Differentially expressed genes detected in antimony-resistant parasites at the basal level (no drug challenge). File name: Supplementary Data 3: Description: Raw data from Venn diagram summarizing the differentially expressed genes detected at the basal level (no drug challenge) per polysome fraction. File name: Supplementary Data 4: Description: Comparison of the translatome Vs. transcriptome (basal level). File name: Supplementary Data 5: Description: Dataset of differentially expressed genes detected in antimony resistant parasites growing under drug challenge. File name: Supplementary Data 6: Description: Raw data from Venn diagram summarizing the differentially expressed genes detected in antimony resistant parasites growing under drug challenge per polysome fraction. File name: Supplementary Data 7: Description: Comparison of the translatome Vs. transcriptome (drug challenge). File name: Supplementary Data 8: Description: Dataset summarizing the normalized read counts by DESeq2’s median of ratios. File name: Supplementary Data 9: Description: List of primers used for the validation of translatome analysis by RT-qPCR. File name: Supplementary Data 10: Description: Comparison between translatomic and proteomic analyses for resistant parasites growing under drug challenge. File name: Supplementary Data 11: Description: Dataset summarizing known and potentially new antimony modulators File name: SupplementaryData 12: Description: Raw data of gene ontology enrichment analysis. File name: Supplementary Data 13: Description: Clustering analysis based on protein homology summarizing changes at the basal level (no drug challenge). File name: Supplementary Data 14: Description: Clustering analysis based on protein co-expression summarizing changes at the basal level (no drug challenge). File name: Supplementary Data 15: Description: Raw data for the glutathione metabolism STRING network dataset summarizing changes under drug challenge. File name: Supplementary Data 16: Description: Manually grouped differentially translated transcripts detected in antimony resistant parasites growing under drug challenge. Supplementary Data 17: Description: Gene variants detected in antimony resistant parasites by using the FreeBayes algorithm. Supplementary Data 18: Description: Comparison between translatomic and variant calling analyses.