List of degenerate primers used for DPMT.

aFor each oligonucleotide, CORE nucleotides are in bold and CLAMP sequences in normal lettering. Underlined codons do not encompass all the possible variability to avoid excessive degeneracy. The sequences used are biased to accommodate the DNA sequences of existing elements. bPrototype plasmid for the given MOB subfamily. cAmplicon size obtained from the prototype plasmid relaxase gene. dNucleotide coordinates of the prototype plasmid contained in the corresponding amplicon. An asterisk indicates that the relaxase gene is encoded in the complementary strand.