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High-depth spatial transcriptome analysis by photo-isolation chemistry

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NIAID Data Ecosystem2026-03-12 收录
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We established a transcriptome profiling method coupled with photo-isolation chemistry (PIC) that allows the determination of expression profiles specifically from photo-irradiated regions of interest. Overall design: We performed PIC to isolate expression profiles from small domain of the E14.5 mouse neural tube. We performed PIC to isolate expression profiles from small domain of HeLa cells or NIH3T3 cells. We performed PIC to isolate expression profiles from each of the major hippocmpal [CA1, CA2 and dentate gyrus (DG) ] in 8 weeks male mice.

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2021-07-29
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