The mntH Gene of Burkholderia cenocepacia influence Motility and Quorum Sensing to Control Virulence

Burkholderia cenocepacia, an important opportunistic pathogen in patients with cystic fibrosis (CF), commonly found in the environment, which mostly utilized N-acylhomoserine lactone (AHL) and cis-2-dodecenoic acid (BDSF) QS systems to control biological functions. And our previous study illuminated the detailed mechanism of B. cenocepacia integrates BDSF and c-di-GMP signals to control virulence. Here we employed Tn5 transposons mutagenesis to identify genes that related to BDSF QS system. One of the most significantly attenuated mutants had an insertion in the mntH gene. Here we showed that a manganese transport protein MntH (Bcam0836) controls QS-regulated phenotypes, including motility, biofilm formation and virulence.