RANKL overexpressing LNCaP cells induced high incidences of bone and soft tissue metastases.

LNCaP-RANKL and LNCaP-neo cells (1×106 cells/50 µl PBS) were inoculated intracardially into 5- to 7-week-old male athymic nude mice (LNCaP-RANKL n = 20; LNCaP-neo n = 15). Metastatic lesions were observed between 2–3 months after injection.*Micro-CT tomography detected bone lesions in limbs, ribs, jaw, and skull determined by X-ray radiography and confirmed by histopathologic staining (H&E).