RNA-sequencing of PDX from small round cell sarcomas

The goal of this study was to profile the transcriptome of different small round cell sarcomas from patient-derived xenografts. We derive unique gene expression associated with each of the fusion proteins driving these sarcomas. Overall design: We obtained fresh-frozen tissue from the PDXs from small round cell sarcomas including Ewing sarcoma, CIC-DUX4 sarcoma and desmoplastic small round cell tumors. After removal, RNA was extracted and processed for RNA-sequencing.