Cotton Fiber Cells: Germplasm Line MD 52ne vs. Germplasm Line MD 90ne

Transcriptional profiling of cotton fiber cells from two cotton germplasm lines, MD 52ne and MD 90ne. Comparison of fiber cell transcription profiles is between the two germplasm lines and over a developmental time-course from 8 to 24 days post anthesis in four day intervals. Overall design: Cotton plants grown in 3-4 row plots of approximately 300-400 individual plants. Bulked fiber samples from multiple plants per each plot represented a biological replication. There were 3-4 spatially distinct plots per cotton germplasm line. Loop microarray hybridization experimental design. Biological replicates: 2 for each germplasm line at each time-point. Technical replicates: 2 for each germplasm line at each time-point (dye-swap).

对两种棉花种质资源线MD 52ne和MD 90ne的纤维细胞转录组进行测序分析。比较两种种质资源线在开花后8至24天，以每4天为一个时间间隔的纤维细胞转录组变化。总体设计：在3-4行的大约300-400株植株的田间种植棉花。每个田块中来自多株植株的纤维样品集合代表一个生物重复。每个棉花种质资源线设有3-4个空间上不同的田块。采用环状微阵列杂交实验设计。生物重复：在每个时间点，每个种质资源线有2个生物重复。技术重复：在每个时间点，每个种质资源线有2个技术重复（染料交换）。