Dataset for article: HPMA polymers as functional excipients in dermal nanoformulations of imiquimod; including the article in pdf

Dataset for article: HPMA polymers as functional excipients in dermal nanoformulations of imiquimod Last updated: 2026-01-09 DOI: 10.5281/zenodo.18195473 ______Contact______ * Eliska Kurfirtova * [e-mail] * [phone] * ORCID: 0000-0003-0691-8667 * Dept. of Organic Technology. Faculty of Chemical Technology, University of Chemistry and Technology, Prague * Technicka 5, 166 28, Prague 6, Czech Republic ______Principal Investigator______ * Jarmila Zbytovska * [e-mail] * [phone] * ORCID: 0000-0003-1541-8488 * Dept. of Organic Technology. Faculty of Chemical Technology, University of Chemistry and Technology, Prague * Technicka 5, 166 28, Prague 6, Czech Republic ______Data manager or custodian______ * Aneta Kalvodova * [e-mail] * [phone] * ORCID: 0000-0002-4224-4340 * Dept. of Organic Technology. Faculty of Chemical Technology, University of Chemistry and Technology, Prague * Technicka 5, 166 28, Prague 6, Czech Republic ______Licence______ *Dataset for HPMA polymers as functional excipients in dermal nanoformulations of imiquimod article by Eliska Kurfirtova is licensed under CC BY 4.0 *licence information: https://creativecommons.org/licenses/by/4.0/ ------------------------------------------------------------------------------------------------ ______About the dataset______ The article focused on the p(HPMA) interaction with the skin barrier, specifically within the stratum corneum (SC), which was studied by confocal microscopy. FTIR analysis confirmed that the polymers increase SC hydration without disrupting lipid organization. As demonstrated by the ex vivo skin permeation study, the smallest p(HPMA) polymer (5 kg/mol) produced the strongest enhancement effect on IMQ delivery into skin tissue. ______Methods of data collection______ *TEWL HW: Aquaflux AF200 (Biox Systems Ltd., UK) Acquisition: minimal acquisition time 30s *FTIR HW: iZ10 (ThermoFisher, USA) with ZnSe ATR crystal (ThermoFisher, USA) acquisition: 64 scans, 2 cm^-1 resolution *Permeation experiments HW: Franz diffusion cells, Thermostated water bath, 60 position mixer (2mag AG, Germany) *DLS HW: Zetasizer Nano ZS (Malwern Instruments, UK) Acquisition: Number od runs: 3, number of scans: 5 *TEM HW: Jeol JEM-1010, Jeol Ltd., Tokyo, Japan Acquisition: accelerating voltage: 80 kV *Confocal microscopy HW: Olympus Fluoview FV1000, using UPLFLN 20x objective, Tokyo, Japan Acquisition: a laser (λ = 488 nm). The used laser strength was 2% *HPLC HW: Prominence LC-20 HPLC (Shimadzu, Tokyo, Japan) equipped with following: reverse-phase column Kinetex® (150 × 4.6 mm, 5 µm, RP C18, 100 Å) (Phenomenex, Torrance, USA); LC-20AD solvent delivery module with DGU-20A degasser; SIL-20AC autosampler; CTO-20AC column oven; SPD-M20A UV/VIS photodiode array detector; CBM-20A communication module *Electrical impedance HW: multimeter LCR 4080 (Voltcraft, Germany) Acquisition: parallel-equivalent mode with an alternating current frequency of 120 Hz ______Methods of data processing______ The FTIR spectra were normalized [0,1] in OriginPro SW using inbuilt normalize function. The exact peak position was evaluated in OriginPro SW using Find Peak function. ---------------------------------------------------------------------------------------------- ______File name structure_____ Example: GACR_HPMA_260107_data summary * RP_XX_YYMMDD_X_Z_000 RP – research proposal XX – article identifier YYMMDD – date of data creation X – type of data Z – measurement details 000 – order of the files in the dataset ______File formats______ * Tabular, TEWL, FTIR, DLS data – converted to ASC ______SW necessary to open files______ * CSV and ASC files can be opened in text editors or Excel (Microsoft, USA) ______Date formats______ * YYYY-MM-DD * HH-MM-SS 24hr format ______Units and abbreviations______ * TEWL data are in (g/m^2/h) * Transdermal permeation and dermal entrapment are in (microg/cm^2) * DLS particle sizes are in (nm) and PDI (-) ------------------------------------------------------------------------------------------------ ______Dataset structure and List of files______ * Summary of data GACR_HPMA_260107_data summary.csv