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Rate of 13C accumulation during a 0.5–10.0 min 13Ci-VHC pulse using 2% (w/w) 13C-NaHCO3 and 13C enrichment at maximum labelling (20–60 min) in the pools of 3-phosphoglycerate (3PGA) and phosphoenolpyruvate (PEP).

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Figshare2015-12-02 更新2026-04-29 收录
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https://figshare.com/articles/dataset/_Rate_of_13_C_accumulation_during_a_0_5_8211_10_0_min_13_C_i_VHC_pulse_using_2_w_w_13_C_NaHCO_3_and_13_C_enrichment_at_maximum_labelling_20_8211_60_min_in_the_pools_of_3_phosphoglycerate_3PGA_and_phosphoenolpyruvate_PEP_/475941
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The PEP/3PGA ratios were calculated from paired observations within each sample. Cultures were pre-acclimated and subjected to a chase of either 5% CO2 (HC) or 0.035% CO2 (LC) of ambient isotope composition. Note that compared to the HC condition, the LC condition resulted in faster and higher labelling of both the 3PGA and PEP pools. Also note that the PEP pool exhibited more rapid and higher labelling than the 3PGA pool. Each replicate experiment (WT n = 3, mutants n = 2) was an independent time series of 7–8 time points sampled ∼0.5–60.0 min after the pulse.
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2015-12-02
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