RNA-Seq of femoral shaft RNA from Catalase knockout (KO) and wild-type (WT) male mice at 12 weeks of age given binge ethanol or control PBS for four consecutive days.

The objective was to determine how binge ethanol and knockout of the catalase gene affect the transcriptome of the femoral shaft in mice. Mice were of two genotypes, Catalase knockout mice (Cat -/-) and wild-type mice (Cat +/+). At 12 weeks of age, mice received either binge ethanol in the form of 3, 3, 4, and 4.5 g ethanol/kg body weight by gavage from a 31.5 % (v/v) ethanol solution on four consecutive days or isovolumetric amounts of PBS. Femoral shaft RNA was isolated. Each sample is a pool of femoral shaft RNA from two male mice.本研究旨在探究过量乙醇暴露与过氧化氢酶（catalase）基因敲除对小鼠股骨干转录组的影响。实验小鼠分为两种基因型：过氧化氢酶基因敲除小鼠（Cat -/-）与野生型小鼠（Cat +/+）。小鼠于12周龄时，连续4天通过灌胃给予31.5%（体积比）乙醇溶液，每日乙醇给药剂量依次为3、3、4、4.5 g乙醇/千克体重；对照组则给予等体积的磷酸盐缓冲液（PBS）。随后分离股骨干组织的RNA，每个样本均为2只雄性小鼠股骨干RNA的混合样品。