Gene expression profiling of mouse CRTC1-MAML2-driven MEC-like tumors

We established a transgenic CRTC1-MAML2 fusion mouse model and crossed it with the MMTV-Cre transgenic mouse line to induce Cre expression and consequently the CRTC1-MAML2 fusion transgene expression in salivary glands. We observed that Cre-induced CRTC1-MAML2 transgene expression caused the formation of salivary gland tumors resembling the histological features of human MEC. Here we performed genome-wide gene expression profiling analysis to characterize the transcriptomic feature of these murine CRTC1-MAML2-driven MEC-like tumors. Gene expression profiling was carried out on CRTC1-MAML2-driven MEC-like tumors (T), matched tumor adjacent normal salivary gland tissues (NAT), and normal salivary gland tissues (N) from the control non-transgene carrier littermates (N). Differentially expressed genes (DEGs) were determined in three comparison groups, including NAT vs. N, T vs. NAT, and T vs. N.