Differentially Expressed microRNAs in Bone Marrow Mesenchymal Stem Cell-derived Microvesicles in Young and Older Rats and Their Effect on Tumor Growth Factor-ß1-mediated Epithelial-Mesenchymal Transition in HK2 cells

miRNA profiles of the Young-MSC-MVs and Old-MSC-MVs were analyzed with a quantitative PCR (qPCR)-based array of the whole rat genome. Further analysis revealed the expression of miR-344a, miR-133b-3p, miR-294, miR-423-3p, and miR-872-3p was significantly downregulated in Old-MSC-MVs than in Young-MSC-MVs (p<0.05).本研究采用基于定量聚合酶链式反应（quantitative PCR，qPCR）的大鼠全基因组表达芯片，对年轻间充质干细胞微囊泡（Young-MSC-MVs）与老年间充质干细胞微囊泡（Old-MSC-MVs）的微小RNA（microRNA，miRNA）表达谱进行了分析。进一步分析显示，相较于Young-MSC-MVs，Old-MSC-MVs中miR-344a、miR-133b-3p、miR-294、miR-423-3p及miR-872-3p的表达水平显著下调（p<0.05）。