Melatonin prolongs survival time of mice with traumatic colon injury by regulating Serpina3n protein expression

Metagenome: This file displays metagenomic sequencing data of intestinal microbiota in the sham and TCI groups.Proteomics: This file displays proteomics data of the intestinal epithelium in the sham and TCI groups.Figure 1: This file displays survival time of the sham and TCI groups within 96 hours. The relative abundances of Shigella, Escherichia and Salmonella between the sham and TCI groups were also exhibited.Figure 2: This file displays the IHC score of Spera3n expression in the intestinal epithelium of the sham and TCI groups.Figure 3: This file displays the following data: (i) The relative cell migration rate of vector, Serpina3n, shNC and shSerpina3n groups (Figure 3c). (ii) The IHC score of Spera3n expression in the intestinal epithelium of WT and Serpina3n KO groups (Figure 3d). (iii) The percent survival of Serpina3n KO mice that received sham or TCI operation and WT mice that received TCI operation (Figure 3f).Figure 4: This file displays the following data: (i) The concentrations of IL-6 and TNF-α in plasma and intestines of WT or Serpina3n KO mice that received sham or TCI operation (Figure 4a). (ii) The concentrations of DAO, D-lactate and endotoxin of mice as in (i) (Figure 4b). (iii) The IHC score of both ZO-1 and occludin expression in the intestinal epithelium of mice as in (i) (Figure 4c). (iv) The relative abundances of Shigella, Escherichia and Salmonella of mice as in (i) (Figure 4d).Figure 5: This file displays the following data: (i) The relative cell migration rate of Caco-2 cells treated with vehicle 0.01 μM, 0.1 μM or 1 μM MLT (Figure 5b). (ii) The percent survival of mice in the sham or TCI groups that were administered with vehicle or MLT (Figure 5d). (iii) The IHC score of Spera3n expression in the intestinal epithelium as in (ii) (Figure 5f).Figure 6: This file displays the following data: (i) The concentrations of IL-6 and TNF-α in plasma and intestines of mice in the sham or TCI group that were administered with vehicle or MLT (Figure 6a). (ii) The concentrations of DAO, D-lactate and endotoxin of mice as in (i). (iii) The IHC score of both ZO-1 and occludin expression in the intestinal epithelium of mice as in (i). (iv) The relative abundances of Shigella, Escherichia and Salmonella of mice as in (i) (Figure 6d).