James R. LaFountain, Rudolf Oldenbourg (2011) CIL:10726, Nephrotoma, Nephrotoma suturalis, primary spermatocyte. CIL. Dataset

Time series of anaphase A and B of meiosis I in primary spermatocytes of the crane-fly Nephrotoma suturalis.
Time-lapse polarization microscopy using a Nikon
Microphot SA, equipped for liquid crystal polarized light microscopy
(LC-PolScope, CRi, Woburn Massachusetts) 60x/1.4 PlanApo oil immersion objective, 1.4 NA oil imm. condenser, with 2.0x zoom lens. Images captured every 30 sec over 60 min using a QImaging Retigo EXi CCD camera. Raw images were processed
using 4-frame algorithm (Shribak and Oldenbourg, 2003). A movie of this time series is included in this grouped set.

本数据集记录了鹤虻（Nephrotoma suturalis）初级精母细胞减数分裂I期中期A和B阶段的时序变化。采用尼康Microphot SA显微镜，配备液晶偏振光显微镜系统（LC-PolScope，CRi，马萨诸塞州沃本），搭载60倍/1.4 PlanApo油浸物镜，1.4 NA油浸聚光镜以及2.0倍变焦镜头进行连续时间间隔摄影。利用QImaging Retigo EXi CCD相机，每隔30秒捕捉一次图像，持续60分钟。原始图像经过四帧算法（Shribak and Oldenbourg，2003年）处理。本组数据集包含该时序变化的视频文件。