Blood miRNA as biomarkers of epilepsy (HIGH vs. LOW)

We analyzed miRNA levels in blood samples using Affymetrix microarrays 4.1 and performed comparative analysis of samples. We performed 3 comparisons: i) control animals vs. Status epilepticus animals, ii) animals which developed first spontaneous seizure around 7 days post stimulation (EARLY), or later after 21days post stimulation (LATE), iii) animals that had high or low number of seizures as determined by seizures number (EARLY vs. LATE).本研究采用Affymetrix微阵列4.1平台检测血液样本中的微小RNA（microRNA，miRNA）水平，并对所有样本开展比较分析。本次分析共设置3组对照比较： i) 对照组动物与癫痫持续状态（Status epilepticus）模型动物； ii) 刺激后约7天出现首次自发性癫痫发作的早期（EARLY）组动物，以及刺激后21天及之后出现首次发作的晚期（LATE）组动物； iii) 依据癫痫发作次数划分为高发作组与低发作组的动物（该比较仅在早期组与晚期组间进行）